What goes into breeding at GTR?

Our breeding program is focused solely on producing the most consistent, reliable, agronomically useful, scientifically advanced, and novel cannabinoid rich feminized seeds available. We are not pollen chuckers. We have never simply picked out two plants we liked and made a cross. 

Everything we create has been made with dedication and intention. When you start 10 or 10,000 of our seeds, you can expect consistently excellent performing genetics. Read on for a look into our breeding program.

F1 Hybrid's

Each one of our lines is produced from two highly inbred parents that have been worked with over many generations. This means every time we release a new seed variety it was many years in the making. We have culled hundreds of thousands of plants that didn't make the cut over the years. They all require the same amount of light, soil, water, and attention from start to finish. 

We start by finding a plant with a cannabinoid or characteristic we like, breed with it, inbreed it, test, test, and inbreed again to lock in the best possible plant we can. We repeat the process on another parent, breeding, inbreeding, selecting, culling, and repeating until we've got another "keeper." Each plant is inbred for at least 3 generations to ensure we have locked in all the best traits we can. From there, we cross the two inbred lines to make  true to type F1 hybrid cannabis seeds.

F1's are celebrated in the plant world for their incredible vigor and homogeny - plant 10 or plant 10,000 and they will all share very similar characteristics. Compare an F1 to a pollen chucker's Zkittlez x Runtz and you'll definitely see a difference.

Feminized Seeds

All of our seeds are feminized, which isn't particularly complicated, but it does take a lot of time. For us this means treating one of our inbred lines with STS to tell a female plant to produce male flowers. The treatments go on over several weeks for maximum pollen production from the male flowers. In some cases the "males" produce hardly any pollen and seed runs are meager - but it in the end it means that we can offer 100% feminized seeds. Every single plant, if tested will show female XX chromosomes. That said we generally see about 1 phenotypically male plant per 4000 plants. 

Breeding Facilities

All of our seeds are produced in fully sealed, climate controlled light deprivation greenhouses. Each of our 18 greenhouses has over 130 individual 1000 Watt HPS lights, ductless heaters and air conditioners, and multiple dehumidifiers. Firing up a house gets the electric meter humming. That said we are proud to host over 2 acres of solar panels.

Feminized seed production must be done in fully sealed houses to prevent cross pollination. In the past we have had neighbors growing non feminized crops of CBD/THC/etc - and any pollen drift from any other variety absolutely destroys our seed runs - and EVERY seed has to be tossed. Those who pickup our seeds can trust what they are buying - is what they are getting.

Plant Testing

In order to release seeds that are pure in any cannabinoid - you must test. For us this meant stepping up and investing in an HPLC machine. This allows us to accurately analyze our varieties to ensure they are rich in whatever cannabinoid we are shooting for be it CBD, THC, CBDV, THCV, etc. When you run a breeding program with plants rich in differing cannabinoids it can be really easy to screw things up. Sending out seeds that have high THC to a hemp farmer can not only lead to serious financial ruin, but also possible jail time. Breeding is complicated and without testing you really have no idea if what you produce is what you say it is. We take pride in delivering.

Breeding with Science

We have developed many firsts in the cannabis industry. From the first commercial CBD autoflower seeds to high CBG seeds, and more recently the first commercial triploid cannabis seeds. These developments took many many years and millions in resources to put into reliably feminized seed form. We also host a Sequel II genomic sequencer and have the largest library of whole sequenced cannabis genome's out there. This machine is at the top of its class for reading genomic sequences and are hosted by very few private institutions. We were asked by the state to sequence a psilocybin mushroom for the first time. While it may just spew out millions of pages of numbers and code - we have been able to identify genomic markers in varieties that allow us to know which plants to keep and which plants to ditch early on. That said it wasn't cheap.....And having the bright minds on staff that know how to run all this fancy machinery is a pleasure but once again ain't free. Not to mention countless hours behind the HPLC machine and Flow Cytometry. While the fancy words may mean little, you wouldn't be picking out the 35% strains you do at a dispensary without the testing.

Triploid Cannabis Seeds

When it comes to breeding seeds, triploids have been perhaps the greatest challenge to us. Not only did we employee a team for years to develop the processes to produce them for commercial sales - the entire process of getting them from concept to seed is far beyond anything we've ever had to do.

Step 1, isolate and inbreed until we find two different lines we like to make an F1 hybrid we are proud of.
Step 2, convert one of the plants to tetraploids. This involves treating dozens of plants over several weeks. The majority do not survive and the few that do take plenty of nurturing to get back to health.
Step 3, test every new shoot that comes off the plant. Using a flow cytometry machine we test every single new shoot that comes off the plant to ensure plants are in fact tetraploids. Plants frequently revert back to diploids rendering them garbage meant for the compost pile. We continue this process for several weeks until we are confident the plants are entirely tetraploids.
Step 4, clone and clone and clone. Once the plant has gained some girth we start taking cuttings that will eventually become the seed baring plants in for our runs.
Steph 5, pollinate. Tetraploid plants don't like to make seeds so the pollination process is long and tedious. We shake pollen over plants daily, from our second independent inbred line, for almost a month on end to impregnate as many possible female flowers as possible. Because tetraploids don't like to make seeds we end up with between 5%-30% of the seeds we would normally end up with after a traditional diploid cross - but pour in the same amount of resources to the growing and pollinating.

Thanks for taking a look into our breeding program. We know you'll appreciate the work.